Isolation and Restriction Mapping of aPseudomonasPlasmid
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چکیده
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the effect of vocabulary instruction through semantic mapping on learning and recall of efl learners
چکیده ندارد.
15 صفحه اولIsolation, characterization and restriction endonuclease mapping of the Petunia hybrida chloroplast DNA.
A procedure is developed for the isolation of intact chloroplast DNA (ctDNA) from Petunia hybrida. The molecular weight, calculated from contour length measurements, is 96.0 +/- 4.5 x 10(6) daltons. This value is in good agreement with the value of 101.2 x 10(6) daltons that was estimated from the electrophoretic mobilities of restriction endonuclease fragments of ctDNA. Analysis of petunia ctD...
متن کاملIsolation and identification of restriction endonuclease BsiSI.
BseBI, an isoschizomer of BstNI (1) has been purified from Bacillus stearothermopilus species. BseBI recognises the sequence 5'...CCWGG...3' (W=A or T) and cleaves between C and W. The enzyme was purified using the following Chromatographic steps: 1. Blue -Sepharose F3GA, 2. Heparin -Sepharose, 3. DEAE-Sepharose. The enzyme was free of contaminating nuclease activity. After 5-fold overdigestion...
متن کاملIsolation and identification of restriction endonuclease SseAI.
SseBI, an isoschizomer of StuI (1) has been purified from Streptomyces species. SseBI recognises the sequence 5' ... AGGCCT ... 3' and cleaves between G and C. The enzyme was purified using the following chromatographic steps: 1. Blue Sepharose F3GA, 2. Heparin-Sepharose. The enzyme was free of contaminating nuclease activity. After 100 fold overdigestion on lambda DNA greater than 95% of the D...
متن کاملLecture 14 Restriction Mapping
Restriction mapping involves cleaving DNA clones at specific sites with enzymes to produce a set of fragments. These fragments have characteristic lengths which together constitute a multiset (a set allowing repeated elements) called the “fingerprint” of the clone. Clones that overlap with each other share common restriction fragments as long as the region of overlap contains at least two enzym...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1982
ISSN: 0002-1369
DOI: 10.1080/00021369.1982.10865446